新加坡南洋理工大学Dr. Xiaogang Liu 5月20日学术报告

发布时间:2010-05-15访问量:94设置

 

    人:新加坡南洋理工大学Dr. Xiaogang Liu

 

Department of Chemistry, NationalUniversity of Singapore, 3 Science Drive 3, Singapore 117543, Singapore

 

报告题目:Colorimetric DNA Detection via Nicking Endonuclease-Assisted Nanoparticle Amplification

 

报告时间:520日下午1430

 

报告地点:FUNSOM实验室208报告室(材料大楼910号楼)

  

Abstract: Important genetic information is encapsulated in the DNA, which is why it is one of the most studied molecules. Such studies may involve DNA detection. Thus, the ability to sense and detect ultra-low concentrations of specific DNA sequences using simple and inexpensive assays is important in many applications ranging from clinical diagnostics such as mutation detection to biodefence applications.  Conventional methods of DNA detection involve the use of radioactive 32P-labeled nucleic acid probes or polymerase chain reactions combined with fluorescent-based analytical methods. Although the latter has high sensitivity of detection, it is not without its drawbacks.  Such include complex handling procedures, easy contamination, high cost, and lack of portability.  To this end, we have developed a novel approach of detecting point mutations or single nucleotide polymorphisms based on nicking endonuclease-assisted nanoparticle amplification.  Their newly designed colorimetric detection system comprises a fixed set of nanoparticle probes, a linker strand that is supposed to hybridise with the target DNA, and a nicking endonuclease enzyme.  The detection system offers a colorimetric detection limit of 0.5 fmol within hours for selected oligonucleotides.  Detection of DNA sequences with a single base mismatch or different lengths (24-mer, 36-mer, 48-mer, and 80-mer) has also been demonstrated.

 

Keywords: Colorimetric detection, DNA, nanoparticle, Single base mismatch

Biodata: Xiaogang Liu was born in Nanchang, Jiangxi Province of China.  He earned his BS degree (1996) in Chemical Engineering from Beijing Technology and BusinessUniversity.  He received his MS degree (1999) in Chemistry from EastCarolinaUniversity in Greenville, North Carolina under the direction of Prof. John Sibert and completed his PhD (2004) in Chemistry at NorthwesternUniversity in Evanston, Illinois under the supervision of Prof. Chad Mirkin.  In the same year he became a postdoctoral fellow in the group of Prof. Francesco Stellacci at MIT.  He joined the faculty of the National University of Singapore in 2006.  His research interests include nanomaterials synthesis and self-assembly, bioinorganic and supramolecular chemistry, and surface science for catalysis, sensors and biomedical applications.

 

 

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