报 告 人：新加坡南洋理工大学Dr. Xiaogang Liu
Department of Chemistry,
报告题目：Colorimetric DNA Detection via Nicking Endonuclease-Assisted Nanoparticle Amplification
Abstract: Important genetic information is encapsulated in the DNA, which is why it is one of the most studied molecules. Such studies may involve DNA detection. Thus, the ability to sense and detect ultra-low concentrations of specific DNA sequences using simple and inexpensive assays is important in many applications ranging from clinical diagnostics such as mutation detection to biodefence applications. Conventional methods of DNA detection involve the use of radioactive 32P-labeled nucleic acid probes or polymerase chain reactions combined with fluorescent-based analytical methods. Although the latter has high sensitivity of detection, it is not without its drawbacks. Such include complex handling procedures, easy contamination, high cost, and lack of portability. To this end, we have developed a novel approach of detecting point mutations or single nucleotide polymorphisms based on nicking endonuclease-assisted nanoparticle amplification. Their newly designed colorimetric detection system comprises a fixed set of nanoparticle probes, a linker strand that is supposed to hybridise with the target DNA, and a nicking endonuclease enzyme. The detection system offers a colorimetric detection limit of 0.5 fmol within hours for selected oligonucleotides. Detection of DNA sequences with a single base mismatch or different lengths (24-mer, 36-mer, 48-mer, and 80-mer) has also been demonstrated.
Keywords: Colorimetric detection, DNA, nanoparticle, Single base mismatch
Biodata: Xiaogang Liu was born in