报告人：Suzie H. Pun 教授
Dept of Bioengineering and Molecular Engineering and Sciences Institute,
University of Washington, Seattle, Washington, USA.
题目：Polymers for Gene Transfer to the Brain
地点：909号楼一楼G会议室(Conference Room G, 909-1F)
Effective gene delivery to the brain remains a significant challenge in translation of nucleic acid therapeutics for neurodegenerative disease. Our laboratory has been developing polymeric materials for in vivo gene transfer. In this presentation, I will discuss some recent materials that we have developed and our findings regarding polymer structure and function as gene transfer agents. We have investigated the role of polymer architecture (linear vs. brush vs cyclic) in intracellular trafficking and gene transfer to cultured cells. We have also synthesized multivalent, peptide based polymers for gene transfer. Peptides that facilitate cell targeting, nucleic acid loading and endosomal escape have been incorporated into water-soluble polymers. Finally, we have prepared environmentally-responsive polymers that can be triggered to release nucleic acid cargo after cellular internalization. These materials were tested for in vivo transfection; the results from these studies and future work will be discussed.
Suzie H. Pun received her Chemical Engineering Ph.D. degree in 2000 from the California Institute of Technology. She then worked as a senior scientist at Insert Therapeutics/Calando Pharmaceuticals for 3 years developing polymeric drug delivery systems before joining the Department of Bioengineering at University of Washington (UW). She is currently the Robert J Rushmer Professor of Bioengineering, an Adjunct Professor of Chemical Engineering, and a member of the Molecular Engineering and Sciences Institute at UW. Her research focus area is in biomaterials and drug delivery and she has published over 75 research articles in this area. Current application areas for her group include biologics delivery to the central nervous system and cancer. For this work, she was recognized with a Presidential Early Career Award for Scientists and Engineers in 2006, the 2014 Inaugural Biomaterials Science Lectureship, and the 2014 Young Investigator Award from the Controlled Release Society.