美国斯坦福大学陈璞博士11月12日上午学术报告

发布时间:2014-11-08访问量:46设置

报告人:陈璞博士

 

美国斯坦福大学医学院

 

题目:Liquid-Based Templated Assembly of Microscale Materials into Diverse Architectures

时间:20141112日(星期三)上午10:00

地点:独墅湖校区910号楼308会议室

 

摘要:

Directed assembly of microscale materials has been motivated by greatdemands in engineering three-dimensional architectures/systems in broad fields including bottom-up tissue engineering, microelectromechanical systems and microphotonics.Conventional manufacturing approaches, such as micromolding and templated self-assembly based on solid moulds, suffer from flexibility in reconfiguring resultingstructures. Here, we demonstrate a bottom-up approach to assemble microscale materials using liquid-based template established by standing waves at the air-liquid surface. This liquid-based template can be dynamically reconfigured in seconds(< 5s), and the assembly on the template can be achieved in a scalable and parallel manner. We illustrate broad applicability of this mechanism by assembling diverse macroscopic structures ranging in area from 100 mm2to 10,000 mm2in an efficient manner from soft matter, rigid bodies and multiple biological samplesin a wide range of sizes from 10 ?m to 2 mm.Assembly of neuron-seeded microcarrier beads into 3D neuralnetworks is significant for neuroscience.However, existing methods lack controllability in the global structure of generated neuralnetwork. Here, we demonstratedcapability of assembling neuron-seeded microcarrier beads into 3D neuronal networkswith controllable global shape.Patterning cell spheroids into various shapes is of significance for tissue engineering due to the capability of spheroid’s fusion into micro-tissues. Existing assembly methods are all based on the strategy of pick-and-place, which suffers from low efficiency. Here, we demonstrate simultaneous assembly of~103cell spheroids (mean size: 200 ?m)into various patterns. Another innovative approach that we present is overcoming challenges of scaffold-free cell assembly where the microscale assembly is limited due to small cell size and requirements for cytocompatibility. We demonstrate the assembly ofa large number(~106)of cells simultaneouslyinto various complex patterns.We also show that the assembled structures can be stabilizedin hydrogelby chemical-and photo-crosslinking after assembly. All these results indicate that liquid-based templated assemblyopens a new paradigm for cost-effective and efficient manufacturing from microscale materials. Especially, it would be a useful biomanufacturing tool that enables various applications in fields such as tissue engineering and neuroscience.

 

个人简介:

学士 2001.09-2005.06 中国 华中科技大学 光信息科学与技术

博士 2005.09-2011.03 中国 华中科技大学 生物医学工程

博士后研究员 2011.11-2014.04 美国 哈佛大学医学院,工学院健康科与技术研究中心;布莱根妇女医院 

博士后学者 2014.05-现在 美国 斯坦福大学医学院;斯坦福金丝雀癌症早期诊断中心 

 

 

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