中科院上海应用物理研究所樊春海研究员11月17日下午学术报告

发布时间:2014-11-14访问量:113设置

报告人:Chun-Hai Fan (樊春海) 研究员

中国科学院上海应用物理研究所

Shanghai institute of applied physics of the Chinese academy of science

 

 

时间20141117星期一下午 4:00

地点909号楼一楼B会议室 (Conference Room B, 909-1F)

题目DNA有序纳米结构的构筑及生物医学应用

 

摘要:

Proteins and nucleic acids are dynamically organized in cells to realize their physiological functions with spatial and temporal orderliness. This type of elegant supermolecular assembly has inspired researchers to create molecular/biomolecular structures with dynamic organization outside of the cells. In particular, DNA nanotechnology has proven to possess extraordinary flexibility and convenience for “bottom-up” construction of exquisite nanostructures with high controllability and precision, which holds great promise in a wide range of applications, e.g., nanofabrication and molecular electronics, in-vivo and in-vitro sensing and drug delivery.

In this talk, I will present several examples of using tetrahedral DNA nanostructures (TDNs) for dynamic organization of biomolecules in vitro. TDNs are three-dimensional (3D) DNA architecture with high mechanical rigidity and structural stability, which are suitable for organization of higher-ordered nanocomplexes and nanodevices. As one of the examples, I will describe how we use TDNs as nanoscale agents for both intracellular imaging and efficient delivery of therapeutic oligonucleotides. We employed single-particle tracking to visualize the internalization of TDNs, and dissect the cell entry pathways of these virus-like nanoparticles. In the second example, I hope to use the way that we employed TDNs to dynamically organize the biosensing interface, and realize macroscopic applications on diagnostics. A critical challenge in surface-based biomolecular detection is the reduced accessibility of target molecules to probes arranged on heterogeneous surface compared to probe-target recognition in homogeneous solution. We improve probe-target recognition properties by introducing a probe bearing a TDN-based platform, which provides significantly enhanced spatial positioning range and accessibility of the probes on surface. We also show the versatility of this TDN-based platform for the detection of a wide range of tumor-associated biomarkers.

 

 

个人简介:

樊春海,男,19743月出生,在南京大学获得生物化学学士(1996)和博士学位(2000),后在加州大学圣巴巴拉分校从事博士后研究 。20041月起任中国科学院上海应用物理研究所研究员,现任物理生物学研究室和上海光源国家科学中心(筹)生物成像中心主任。2004年入选中国科学院百人计划,2007获得国家杰出青年基金,2011年任科技部重大科学研究计划(纳米)首席科学家。兼任美国化学会ACS Applied Materials & Interfaces副主编,Scientific Reports, Advanced Healthcare Materials, Journal of Materials Chemistry, Electroanalysis, Particle等杂志编委,Advanced Materials客座编辑。主要研究方向为生物传感器、DNA纳米技术与DNA计算和生物光子学。已发表论文近300篇,论文引用10000余次。主要获得荣誉包括:国际电化学学会会士(ISE fellow)、全球高引用研究者(High Cited Researchers 2014, Thomson Reuters)、第十二届中国青年科技奖、中国科学院杰出青年科学家奖、科技部创新人才推进计划-中青年科技创新领军人才等。

 

联络人: 李有勇 教授

 

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