德国图宾根大学Dr. Fajun Zhang 9月17日上午学术报告

发布时间:2015-09-11访问量:45设置

报告人:Dr.Fajun Zhang

Institut für Angewandte Physik, Universit?t Tübingen, Germany

题目:Tuning Interactions in Protein Solutions towards Controlled Protein Crystallization

时间09:30 am, Sep. 17th Thursday

地点Conference Room B, BLDG 909-1F

 

摘要

Non-specific protein-protein interactions in aqueous solution play a crucial role on protein crystallization and protein-aggregation related diseases, such as cataracts, and sickle cell anemia [1]. A comprehensive understanding of interactions in protein solutions is still a challenge. In this talk, I will first show an efficient way to tune interactions and phase behavior in protein solutions using salts, in particular with trivalent salts. For example, negatively charged globular proteins at neutral pH in the presence of multivalent metal ions undergo a “reentrant condensation (RC)” phase behavior [2,3]. Small angle X-ray and neutron scattering (SAXS/SANS) have been used to characterize the effective interactions under various conditions. In the second part, I will discuss the nonclassical mechanisms of protein crystallization based on our recent work. We show that crystallization near phase boundaries follows different mechanisms [4,5]. Real-time SAXS studies provide the direct evidence for a two-step nucleation process, the role and the structural signature of the metastable intermediate phases in the nonclassical process of protein crystallization [5].

[1] Gunton, J. D.; Shiryayev, A.; Pagan, D. L. Protein Condensation-kinetic pathways to crystallization and disease; Cambridge University Press: New York, 2007.

[2] Zhang, F. et al. Phys. Rev. Lett. 2008, 101, 148101 and Proteins 2010, 78, 3450-3457.

[3] Roosen-Runge, F. et al. J. Phys. Chem. B 2013, 117, 5777. Sci. Rep. 2014, 4, 7016

[4] Zhang, F. et al. J. Appl. Cryst. 2011, 44, 755; Soft Matter 2012, 8, 1313 and Faraday Discuss. 2012, 159, 313.

[5] Sauter, A. et al. J. Am. Chem. Soc. 2015, 137, 1485 and Faraday Discuss. 2015, 179, 41.

 

简介:

http://www.physik.uni-tuebingen.de/institute/institut-fuer-angewandte-physik/forschung/physik-der-molekularen-und-biologischen-materie/biophysics-and-nanoscience.html

 

 

联系人: Prof. Steffen Duhm

 

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