Real-Time PCR Detection System

time:2017-07-13Hits:29设置

A real-time polymerase chain reaction (Real-Time PCR), also known as quantitative polymerase chain reaction (qPCR), is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR), and semi-quantitatively, i.e. above/below a certain amount of DNA molecules (semi quantitative real-time PCR).


Instrument name

Real-Time PCR Detection System

Model number

CFX Connect

Manufacturer

BIO-RAD

Specifications

1. Channel number: 3; 2 target genes and 1 FRET channels can 

     be detected simultaneously.

2. Excitation light source: high intensity LED lamp; Detector: PTD.

3. Excitation / emission wavelength range: 450-580 nm.

4. Sample number: 96 well; Sample carrier: 96 well plate, pipe, 

    PCR pipe.

5. Sample Volume: 1-50?l.

6. Suitable for a variety of fluorescence methods: Taqman

     Molecular Beacon, FRET Probe, SYBR Green I.

7. Temperature Gradient: With 8 dynamic temperature gradient 

     PCR function.

8. Maximum Block Ramp Rate: 5 °Cs

9. Temperature Range: 0-100 °C; Temperature Accuracy: ±0.2 °C;

    Temperature Uniformity: ±0.4 °C.

10. Dynamic Range: no less than 10 orders of magnitude.

11. Sensitivity: to detect single copy gene in human genome. 

Applications and Uses

The fluorescent quantitative PCR is widely used, which can be

used in

1. Absolute quantification of nucleic acids (DNA or RNA)

2. Analysis of gene expression level

3. detection of gene mutation

4. GMO detection and product specificity analysis

5. Genotyping: for example, SNP detection, methylation detection

    and other research areas.

Contacts

Xiaofang Tan

Contact number

65884616

E-mail

xftan@suda.edu.cn


Editor: Xiaofang Tan





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