A real-time polymerase chain reaction (Real-Time PCR), also known as quantitative polymerase chain reaction (qPCR), is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR), and semi-quantitatively, i.e. above/below a certain amount of DNA molecules (semi quantitative real-time PCR).
Real-Time PCR Detection System
1. Channel number: 3; 2 target genes and 1 FRET channels can
be detected simultaneously.
2. Excitation light source: high intensity LED lamp; Detector: PTD.
3. Excitation / emission wavelength range: 450-580 nm.
4. Sample number: 96 well; Sample carrier: 96 well plate, pipe,
5. Sample Volume: 1-50?l.
6. Suitable for a variety of fluorescence methods: Taqman,
Molecular Beacon, FRET Probe, SYBR Green I.
7. Temperature Gradient: With 8 dynamic temperature gradient
8. Maximum Block Ramp Rate: 5 °C／s；
9. Temperature Range: 0-100 °C; Temperature Accuracy: ±0.2 °C;
Temperature Uniformity: ±0.4 °C.
10. Dynamic Range: no less than 10 orders of magnitude.
11. Sensitivity: to detect single copy gene in human genome.
Applications and Uses
The fluorescent quantitative PCR is widely used, which can be
used in ：
1. Absolute quantification of nucleic acids (DNA or RNA)
2. Analysis of gene expression level
3. detection of gene mutation
4. GMO detection and product specificity analysis
5. Genotyping: for example, SNP detection, methylation detection
and other research areas.
Editor: Xiaofang Tan